biochemical catalysts in life and industry by Jean Effront

By Jean Effront

Initially released in 1917. This quantity from the Cornell college Library's print collections used to be scanned on an APT BookScan and switched over to JPG 2000 structure through Kirtas applied sciences. All titles scanned disguise to hide and pages may possibly comprise marks notations and different marginalia found in the unique quantity.

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Thrombin was discovered by Buchanan in 1835. This enzyme, or rather one of the constituent parts of this enzyme, is found in abundance in the white corpuscles of the blood. I t is also found in the red corpuscles, though in much smaller proportion, and in many tissues. I t exists in the lymph, which has approximately the same composition as blood, and in pus, which is a sort of serum in which float the white corpuscles that have undergone fatty degeneration. Much discovered the presence of thrombin in staphylococcus aureus, and Higuchi has, by the aid of physiological salt solution, isolated this enzyme from the soft mass of the placenta.

With certain precautions, and by centrifuging the blood thus obtained, a plasma is obtained which remains liquid for a month or more. However, this goose plasma, with addition of even a very small quantity of the juice of bruised tissues, at once coagulates. 4. To prepare a rabbit plasma capable of being preserved several hours without coagulating, advantage is taken of the retarding influence which paraffin exerts on the appearance of thrombin. Withdrawing the blood by means of paraffined tubes and centrifuging in receptacles likewise paraffined, there is obtained a plasma which, preserved in vessels coated with this substance, keeps liquid for four or five hours, sometimes even for twenty-four or thirty hours.

One of the constituent products of thrombin, thrombokinase, is found deeply imprisoned in the leucocytes. In the normal state, in the circulating stream, the white corpuscles, by a kind of adaptation to the nature of the walls of the bloodvessels in which they circulate, do not give up their enzyme. Its appearance outside corresponds to a certain excitation, to a weakening, or even to the death of the cells. Under the influence of various causes, and especially under the influence of contact other than that of the wall of the blood-vessels, contact which modifies the surface tension of the blood corpuscles, there is a n abundant excretion of the active substance, not because the cells are dislocated or broken, but simply because a certain mechanical excitation results from such contact.

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