BSc in Medical Technology (Laboratory Medicine) Part-II by Monir Ahmed

By Monir Ahmed

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Morphology of RBC and WBC can easily be identified. 2. It prevents the clumping of the platelets, thus making the platelet count more accurate. 3. Bone marrow study can be done by using 2% solution of EDTA. 4. It does not interfere in the most of the bio-chemical tests. *Q-3. What are the criteria of a good film? Write down the steps of preparation of a blood film. Answer. The criteria of a good film: 1. It should be uniformly thick but not too thick. 2. The margin should be even and should not touch the side of the slide.

Hepatitis C virus d. Hepatitis D virus/ Delta virus e. Hepatitis E virus H. Liver biopsy I. Miscellaneous 1. Serum α fetoprotein 2. Serum ferritin level. 3. Total iron binding capacity 4. Serum α1- antitrypsin ***Q-3. Name the common ova found in stool with figure. Write down the indication of stool culture. Answer: The common ova found in the stool are as follows- Ankylostoma duodenale AL (Fertilized ova) AL (unfertilized ova) Enterobious vermicularis Taenia spp. Hymenolepsis nana Trichuris trichiura Indication of stool culture: 1.

Mordanting: The stain is drained off and washed with Lugol’s iodine solution or Grams iodine solution. More iodine solution is poured on the film and kept for 2 minutes. 4. Decolourization: The iodine solution is washed off with alcohol or acetone and then washed with water. The alternate washings with alcohol and water are repeated till the colour ceases to come out of the film. (Acetone does this more quickly than alcohol, so care should be taken not to use acetone for a longer period. Serous and mucoid materials are more difficult to decolourize than saline suspensions and required a longer exposure to the decolourizing agent).

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