Atlas of Confocal Laser Scanning In-vivo Microscopy in by Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD,

By Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)

Confocal microscopy with laser scanning know-how yields in-vivo photographs of ocular and ocular adnexal surfaces which are so awesome that they rival histology when it comes to quality.This precise atlas and textbook demonstrates basic in-vivo anatomy of the cornea, limbus and conjunctiva, quantifies quite a few mobile buildings utilizing cell-density calculations and establishes correlations among novel optical sections of assorted ailments of the ocular floor and medical findings. moreover, it helps the translation of novel high-magnification optical sections by means of evaluating corneal and conjunctival imprint cytology with in-vivo photos and describes early inflammatory alterations in corneal grafts, in addition to corneal conjunctivalisation in limbal stem mobile deficiency, corneal dystrophies or infections, flap interface and margin features after laser in-situ keratomileusis (LASIK). furthermore, it instructs the reader approximately diagnostic and healing follow-up options and offers a quick creation to purposes in different fields reminiscent of dentistry and ear, nostril and throat surgery.

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Extra info for Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology

Example text

Scanning laser reflex (arrow) on the cornea (argon laser/Heidelberg Retina Angiograph Classic) Fig. 2 Normal tear film 32 Chapter 5 Confocal Laser Scanning In Vivo Microscopy Replacing the contact system in the confocal laser scanning microscope with a dry objective lens, combined with an 80–90% absorption gray filter to decrease laser intensity (Fig. 1), enables the fine structure of the tear film to be imaged (Fig. 2). The rapid imaging sequence in the device also permits dynamic processes to be recorded [51, 77, 88].

C Lens fibers (posterior capsule opacification) after lens refilling with silicon polymer Confocal Laser Scanning In Vivo Microscopy 5 The layered structure of the ocular coats can be visualized with high contrast using the RCM attachment with the HRT II and, because of the good quality of depth resolution, can be imaged in optical sections a few micrometers thick. The description of the normal in vivo microanatomy will proceed inward, beginning with the outer structures. 1 Normal Anatomy The preocular tear film with its complex fluid structure bathes the cornea and conjunctiva.

The rapid imaging sequence in the device also permits dynamic processes to be recorded [51, 77, 88]. a Fig. 3 Normal and pathological tear film. a Normal tear film, noncontact examination. 2 Pathological Findings b up time; the surface of the superficial cells is visible in the area of the dry spot b Fig. 1 Superficial Cells (Up to Approximately 50 mm in Diameter) In the case of the most superficial epithelial cells, bright cell borders and a dark cell nucleus and cytoplasm are readily visualized on confo- cal laser scanning microscopy.

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